Identification and characterization of the simian varicella virus uracil DNA glycosylase
β Scribed by C. V. Ashburn; W. L. Gray
- Publisher
- Springer Vienna
- Year
- 1999
- Tongue
- English
- Weight
- 252 KB
- Volume
- 144
- Category
- Article
- ISSN
- 1432-8798
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An optimized reaction condition for amplification of influenza A virus RNA, by thermus thermophilus (Tth) DNA polymerase-based PCR, incorporating uracil N glycosylase (UNG) and dUTP in the reaction has been determined. dUTP could not be substituted for all dTTP sites when UNG was present in the reac
## Abstract Investigation of varicellaβzoster virus (VZV) DNA in 20 healthy children with varicella vaccination was carried out by using the polymerase chain reaction (PCR) and nested double PCR. Samples of peripheral blood mononuclear cells (PBMC) and throat swabs were simultaneously collected 3 t