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Identification and characterization of microsatellite markers in the Chagas disease vector Triatoma dimidiata

✍ Scribed by Jennifer M. Anderson; James E. Lai; Ellen M. Dotson; Celia Cordon-Rosales; Carlos Ponce; Douglas E. Norris; C. Ben Beard

Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
178 KB
Volume
1
Category
Article
ISSN
1567-1348

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✦ Synopsis

Triatoma dimidiata, one of the major vectors of Chagas disease in Central America, is found in both domestic and peri-domestic habitats. Questions concerning population boundaries, infestation rates, insecticide resistance, and geographic dispersal of triatomine bugs persist and may be resolved using genetic markers such as microsatellites. Microsatellites are short tandem repeats found dispersed throughout a genome and can be useful for genotypic identification. We developed a plasmid library from the genomic DNA isolated from a single T. dimidiata adult collected in Guatemala. Ten thousand clones were screened using a probe consisting of nine microsatellite oligonucleotides. Eight loci appear polymorphic among populations found in Guatemala, Honduras, and Mexico, and thus are potentially useful for population genetic applications.

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