Identification and characterization of cysteine proteinases ofTrypanosoma evansi
โ Scribed by S. C. Yadav; R. Kumar; S. Kumar; U. Tatu; R. K. Singh; A. K. Gupta
- Publisher
- Springer-Verlag
- Year
- 2011
- Tongue
- English
- Weight
- 242 KB
- Volume
- 109
- Category
- Article
- ISSN
- 1432-1955
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Four different cysteine proteinases from a cultured human epidermal cell line (NCTC 2544) were partially purified and characterized. The biggest hydrolase was an endoaminopeptidase with the molecular weight of several hundred kilodaltons. It was a glycoprotein and had an almost neutral pH optimum. T
Protein inhibitors capable of inhibiting BCP (Bombyx cysteine proteinase) were found in the larval-pupal hemolymph of Bombyx mori. Two forms of the inhibitors, named BCPI (BCP inhibitor) a and BCPI b, were purified from the pupal hemolymph by heat treatment and column chromatographies on CMcellulose