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Identification and Characterization of an Antigen Recognized by Monoclonal Antibody TRA 54 in Mouse Epididymis and Vas Deferens

✍ Scribed by Kélen F. Arrotéia; Paulo P. Joazeiro; Áureo T. Yamada; Hiromitsu Tanaka; Yoshitake Nishimune; Luís. A. V. Pereira

Book ID: 118761501
Publisher: American Society of Andrology
Year: 2004
Tongue: English
Weight: 657 KB
Volume: 25
Category: Article
ISSN: 1939-4640
DOI: 10.1002/j.1939-4640.2004.tb03161.x

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## Abstract This paper describes an improved scheme for the identification of antigens in crude extracts recognized by specific antibodies when analyzed by a combination of two‐dimensional gel electrophoresis and immunoblotting. First, protein components in gels are electrophoretically transferred