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Identification and behavior of the precipitating BLV antibodies in sera of leukotic cattle

✍ Scribed by W. Matthaeus; O. R. Kaaden; B. Frenzel; E. Schlotterer

Publisher
John Wiley and Sons
Year
1978
Tongue
French
Weight
771 KB
Volume
22
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

Precipitating bovine leukemia virus antibodies isolated from bovine leukemic sera by ion‐exchange chromatography, gel filtration and affinity chromatography were identified as immunoglobulins of the IgG~1~ subclass and IgA class. They proved to be different with regard to molecular size and electrical charge. Immunoglobulins IgG~2~ and IgM as well as low‐molecular components ranging in the micro‐globulin regions (<4S) failed to precipitate BLV antigens. Individual bovine leukemic sera showed differing precipitating activities against p24 and gp69 antigens. Precipitating monospecific gp69 antibodies represented exclusively the IgG~1~ subclass. Some unidentified antigens in disrupted BLV preparations showed serological relationship to p24 and gp69, respectively.

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