ICP-MS-Based strategies for protein quantification

✍ Scribed by Meng Wang; Wei-Yue Feng; Yu-Liang Zhao; Zhi-Fang Chai

• Book ID: 102943531
• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 535 KB
• Volume: 29
• Category: Article
• ISSN: 0277-7037
• DOI: 10.1002/mas.20241

✦ Synopsis

Abstract

In the post‐genomics era, proteomics has become a central branch in life sciences. An understanding of biological functions will not only rely on protein identification, but also on protein quantification in a living organism. Most of the existing methods for quantitative proteomics are based on isotope labeling combined with molecular mass spectrometry. Recently, a remarkable progress that utilizes inductively coupled plasma‐mass spectrometry (ICP‐MS) as an attractive complement to electrospray MS and MALDI MS for protein quantification, especially for absolute quantification, has been achieved. This review will selectively discuss the recent advances of ICP‐MS‐based technique, which will be expected to further mature and to become one of the key methods in quantitative proteomics. © 2009 Wiley Periodicals, Inc., Mass Spec Rev 29:326–348, 2010