I-A Mutation resulted in a selective loss of an antigen-specificIr gene function

✍ Scribed by Lin, C. Shirley ;Rosenthal, Alan S. ;Hansen, Ted H.

• Book ID: 102448693
• Publisher: Wiley (John Wiley & Sons)
• Year: 1981
• Tongue: English
• Weight: 336 KB
• Volume: 16
• Category: Article
• ISSN: 0275-3723
• DOI: 10.1002/jsscb.1981.380160202

✦ Synopsis

Abstract

The immune responses to several antigens were compared in the I‐A mutant mouse strain B6.C‐H‐2^__bm__12^ and the wild‐type strain C57BL/6. With a lymph node cell proliferation assay, the response to two of these antigens, beef insulin and (TG)A‐L, was demonstrated to be controlled by a gene in the I‐A^b^ region. B6.C‐H‐2^__bm__12^ mice failed to respond to beef insulin, while their responses to (TG)A‐L, DNP‐OVA and PPD were comparable with those of the wild‐type strain C57BL/6. Taken together with previous studies, these data suggest that the product of a single pleiotropic I‐A gene, an la molecule, functions as a histocompatibility, la, and MLR antigen, as well as a necessary component for Ir gene function. Furthermore, the data reported here demonstrate that la molecules have multiple functional “Ir determinants,” one of which has been altered in the B6.C‐H‐2^__bm__12^ mutant. The B6.C‐H‐2^__bm__12^ mice, therefore, represent a powerful analytical tool for the understanding of the cellular and molecular basis for Ir gene control of the immune response.