Hypothermia-induced platelet aggregation in human blood in anin vitro model: The dominant role of blood-material interactions

✍ Scribed by Hall, Matthew W. ;Solen, Kenneth A.

• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 439 KB
• Volume: 59
• Category: Article
• ISSN: 0021-9304
• DOI: 10.1002/jbm.1266

✦ Synopsis

Abstract

Hypothermia‐induced platelet aggregation (HIPA) with or without neutrophil involvement may cause neurologic dysfunction during hypothermic surgery. We report the use of a previously developed model to study the contributions of several surfaces, surface area, shear rate, and blood–material exposure time to HIPA. Heparinized (1.5 u/mL) human blood was quenched to 24°C and passed (0.5 mL/min) through a 75‐cm long 1/32” ID tubing of polyvinylchloride (PVC), polyethylene (PE), polyurethane (PU), Teflon‐FEP, or heparin (Duraflo)‐coated PVC. The number of aggregates was measured by a light‐scattering method, and the concentration of occlusive aggregates was assessed using constant‐pressure filtration (50 mmHg). No differences were seen among PVC, PE, PU, or Teflon‐FEP. The heparin‐coated PVC tubing produced fewer occlusive aggregates, and heparin leaching from the coating was not the cause of the decrease in occlusive aggregates. Increasing surface area increased the number of aggregates, and increasing shear rates decreased the occlusiveness of those aggregates. ©2001 Wiley Periodicals, Inc. J Biomed Mater Res 59: 528–534, 2002