Hypothalamo-pituitary hypothyroidism detected by neonatal screening for congenital hypothyroidism using measurement of thyroid-stimulating hormone and thyroxine

A highly sensitive sandwich enzyme immun

A highly sensitive sandwich enzyme immunoassay of human thyroid-stimulating hormone in dried blood on filter paper discs for mass-screening of neonatal hypothyroidism

✍ Seiichi Hashida; Koichiro Tanaka; Takeyuki Kohno; Eiji Ishikawa; Hozo Umehashi; 📂 Article 📅 1987 🏛 John Wiley and Sons 🌐 English ⚖ 575 KB

A sensitive sandwich enzyme immunoassay of human thyroid-stimulating hormone (hTSH) in dried blood on filter paper discs is described for mass-screening of hypothyroidism in the neonates. One 3 mm filter paper disc per assay, on which 2.7 PI of neonatal blood had been dried, was incubated in buffer