Hypericum perforatum—Chemical profiling and quantitative results of St. John's Wort products by an improved high-performance liquid chromatography method
✍ Scribed by M. Ganzera; J. Zhao; I.A. Khan
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 152 KB
- Volume
- 91
- Category
- Article
- ISSN
- 0022-3549
No coin nor oath required. For personal study only.
✦ Synopsis
The analysis of ¯avonoids, naphthodianthrones, and the phloroglucinol derivative hyperforin in H. perforatum is described in this article. In a 35-min HPLC run nine major compounds could be identi®ed and baseline separated in the methanolic plant extracts. For an optimum separation the mobile phase consisted of 10 mM ammonium acetate buffer (pH 5.0) and an acetonitrile/methanol mixture; a Synergi MAX-RP 80 A Ê column (C-12 material) was used as stationary phase. Detection was performed at 270 nm, and the identity of the compounds was con®rmed in an LC-MS experiment. Commercial St. John's Wort products were analyzed and qualitative and quantitative results are discussed.