Nonradioactive Quantification of Glucoco
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S. Roux; B. Terouanne; H. Defacque; I. Vachier; J. Loubatiere; J.C. Nicolas
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Article
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1995
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Elsevier Science
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English
β 567 KB
We describe a method for relative quantification of specific mRNA using a nonradioactive assay based on DNA strand competition between identical sequences of biotin- and fluorescein-labeled amplicon (probe) and unlabeled amplicon (target) during hybridization. As the target quantity increased, that