Hydroxycoumarins as sensitizers and reactants of chemiluminescent oxidative reactions

✍ Scribed by Slawinska, Danuta ;Slawinski, Janusz

• Book ID: 101716077
• Publisher: John Wiley and Sons
• Year: 1989
• Weight: 283 KB
• Volume: 4
• Category: Article
• ISSN: 0884-3996
• DOI: 10.1002/bio.1170040132

✦ Synopsis

Chemiluminescence (CL) of 7-hydroxycoumarin, umbelliferone, a strongly fluorescing compound, the precursor of photoactive furocoumarins widely spread in plant kingdom and used in dye lasers has been examined in four oxidative systems producing active oxygen species: Ahorseradish peroxidase + H202 + buffers pH = 3-10; B -K3Fe (CN16 + H202 + buffers pH = 4-12; C -NaOCl + buffer pH = 3-10.8; and D -HCHO + H202 + K2C03 (Na2C03), the so-called TrautzSchorigin reaction. In all these systems a 10-1000-fold increase of CL intensity and quantum yield and an appearance of the blue emission band (h,,, = 460480 nm), typical of the fluorescence of hydroxycoumarin used have been observed. Spectrophotometric and fluorimetric measurements of the systems A-D indicate that the tested hydroxycoumarinscoumarin, umbelliferone and 6-glucoside umbelliferone or esculin undergo slow (systems A and B) and fast (systems C and D) consumption. leading t o the degradation of coumarins. These findings suggest that efficiently fluorescing hydroxycoumarins may act both as sensitizerssecondary emitters of CL and/or reactants undergoing oxidative degradation.