Hydroxyapatite formed on/in agarose gel induces activation of blood coagulation and platelets aggregation
✍ Scribed by Shin-ichiro Arimura; Ko-ichi Kawahara; Kamal Krishna Biswas; Kazuhiro Abeyama; Masashi Tabata; Toru Shimoda; Daisuke Ogomi; Michiya Matsusaki; Shinya Kato; Takashi Ito; Kazumasa Sugihara; Mitsuru Akashi; Teruto Hashiguchi; Ikuro Maruyama
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 204 KB
- Volume
- 81B
- Category
- Article
- ISSN
- 1552-4973
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✦ Synopsis
Abstract
We reported earlier that hydroxyapatite (HA) formed on/in agarose gels (HA/agarose) produced by alternate soaking process is a bone‐filling material possessing osteoconductive and hemostatic effects. This process could allow us to make bone‐like apatite that was formed on/in organic polymer hydrogel matrices. Here, we investigated the mechanism of hemostasis induced by HA/agarose and found that HA/agarose, but not agarose or HA powder, significantly shortened activated partial thromboplastin time (APTT). While HA/agarose did not show significant platelet aggregation, it markedly enhanced adenosine diphosphate (ADP)‐induced platelet aggregation. Moreover, Western blot analysis revealed selective adsorption of vitronectin onto HA/agarose. We also observed marked differences between HA powder and HA/agarose in their XRD patterns. The crystallinity of HA powder was much higher compared to that of HA/agarose. Furthermore, 50–100 nm of tube‐form aggregations was observed in HA powder on the other hand 100–200 nm of particles was observed in HA/agarose by SEM observation. Thus 100–200 nm of low crystallized particles on the surface structure of HA/agarose may play an important role in hemostasis. Our results demonstrated a crucial role of HA/agarose in the mechanism of hemostasis and suggested a potential role for HA/agarose as a bone‐grafting material. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2006