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Hydrophobic vs coulombic interactions in the binding of steroidal polyamines to DNA

✍ Scribed by James G. Muller; Mabel M. P. Ng; Cynthia J. Burrows

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
526 KB
Volume
9
Category
Article
ISSN
0952-3499

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✦ Synopsis

Seven new steroidal polyamines derived from bile acids, either lithocholic or deoxycholic acid, have been studied as DNA-binding agents using four complimentary methods: an ethidium displacement assay, observed changes in the thermal denaturation of poly[d(AT)], effects on hyperchromicity of DNA, and circular dichroism. In addition, modelling studies were conducted to examine the electrostatic surface potential of the polycations. The results point to a key role for a large hydrophobic surface area on the steroid in addition to the Coulombic attraction by ammonium and guanidinium groups on the steroid interacting with the polyphosphate backbone.

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