Hydrophobic-interaction chromatography of glycosaminoglycuronans: The contribution of N-acetyl groups in heparin and heparan sulfate to the affinity for hydrophobic gels, and variety of molecular species in beef-kidney heparan sulfate
✍ Scribed by Kinzo Nagasawa; Akira Ogamo; Hideki Uchiyama; Kimiyo Matsuzaki
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 635 KB
- Volume
- 111
- Category
- Article
- ISSN
- 0008-6215
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✦ Synopsis
Contribution of N-acetyl groups in heparin and heparan sulfate to their affinity for hydrophobic gels was examined by use of a series of semi-synthetic, N-acetylated, hog-intestinal heparins, a whale-intestinal heparin, and a beef-kidney heparan sulfate. Chromatography on Phenyl-Sepharose CL-4B in 3.8-1.0M ammonium sulfate-10mM hydrochloric acid indicated that an increasing N-acetyl content, which is correlated to a decreasing N-sulfate content, results in a marked increase in the affinity for the gels. The variety of molecular species in beef-kidney heparan sulfate, previously fractionated by conventional chromatographic procedures, was demonstrated by separating further, by hydrophobic-interaction chromatography, the polysaccharide into several fractions composed of molecular species distinctly different in N-acetyl and sulfate content, and in molecular size.