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Hydrophilic interaction chromatography

โœ Scribed by Mariana Roberto Gama; Raquel Gomes da Costa Silva; Carol H. Collins; Carla B.G. Bottoli


Book ID
116927114
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
493 KB
Volume
37
Category
Article
ISSN
0165-9936

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We describe a chromatographic procedure for the removal of sodium dodecyl sulfate (SDS) from proteins isolated by electroelution. It involves chromatography of electroeluates on poly(2-hydroxyethyl-aspartamide) silica, a support initially developed for hydrophilic interaction chromatography. The ele