An increasingly popular analytical method, hydrophilic interaction chromatography (HILIC) has the ability to retain and separate polar compounds that are often difficult to analyze by reversed-phase high-performance liquid chromatography (HPLC) or other analytical methods. Offering a comprehensive review, this book enables readers to develop a fundamental understanding of how HILIC works and then apply that knowledge to develop and implement a variety of practical applications.
Hydrophilic Interaction Chromatography begins with discussions of HILIC retention mechanisms, stationary phases, and general method development. This sets the foundation for the book's extensive coverage of applications. The authors address unique separation challenges for bioanalytical, environmental, pharmaceutical, and biochemical applications. Moreover, there is a thorough discussion of HILIC in two-dimensional chromatography.
With contributions from leading analytical scientists who have extensive experience in HILIC as well as HPLC, Hydrophilic Interaction Chromatography serves as a practical guide for researchers, featuring:
- Detailed examples of HILIC methods and development approaches
- Thorough explanations of retention mechanisms and the impact of stationary phase and mobile phase properties on separations
- Step-by-step guidance for developing efficient, sensitive, and robust HILIC methods
- References to the primary literature at the end of each chapter
Hydrophilic Interaction Chromatography is written for scientists who use or develop analytical methods for the separation of polar compounds. In particular, these researchers will discover how HILIC can be used to analyze and better understand the composition of pharmaceutical, bioanalytical, biochemical, chemical, food, and environmental samples.
Content:
Chapter 1 Separation Mechanisms in Hydrophilic Interaction Chromatography (pages 1–41): David V. McCalley
Chapter 2 Stationary Phases for Hilic (pages 43–85): Mohammed E. A. Ibrahim and Charles A. Lucy
Chapter 3 Hilic Method Development (pages 87–110): Yong Guo and Xiande Wang
Chapter 4 Pharmaceutical Applications of Hydrophilic Interaction Chromatography (pages 111–168): Bernard A. Olsen, Donald S. Risley, V. Scott Sharp, Brian W. Pack and Michelle L. Lytle
Chapter 5 Hydrophilic Interaction Chromatography (HILIC) for Drug Discovery (pages 169–193): Alfonso Espada and Mark Strege
Chapter 6 Advances in Hydrophilic Interaction Chromatography (HILIC) for Biochemical Applications (pages 195–217): Fred Rabel and Bernard A. Olsen
Chapter 7 HILIC?MS for Targeted Metabolomics and Small Molecule Bioanalysis (pages 219–238): Hien P. Nguyen, Heather D. Tippens and Kevin A. Schug
Chapter 8 HILIC for Food, Environmental, and Other Applications (pages 239–264): Michael A. Koupparis, Nikolaos C. Megoulas and Aikaterini M. Gremilogianni
Chapter 9 Theory and Practice of Two?Dimensional Liquid Chromatography Separations Involving the HILIC Mode of Separation (pages 265–305): Stephen R. Groskreutz and Dwight R. Stoll