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Hydrolysis of unordered xanthan in solution by fungal cellulases

✍ Scribed by Ian W. Sutherland

Publisher: Elsevier Science
Year: 1984
Tongue: English
Weight: 709 KB
Volume: 131
Category: Article
ISSN: 0008-6215
DOI: 10.1016/0008-6215(84)85407-5

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✦ Synopsis

After removal of ions, Xanthomonas campestris polysaccharides (xanthan) could be dissolved in distilled water to give the unordered conformation.

In this condition, the polymers were susceptible to random degradation by fungal cellulases at 40-50". The products, including D-glUCOSe, oligosaccharides, and larger fragments, could be separated by gel-permeation chromatography.

The pattern of fragmentation depended on the substrate and on the enzyme preparation used. Some of the oligosaccharides have been partially characterised. The system is of particular interest in that the absence of salts is required for enzyme activity and the xanthan molecule is only susceptible in the unordered conformation. The cellulase systems provide a means of producing fragments of various d.p. from xanthan preparations in reasonable yield.

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