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Hydrolysis of p-nitrophenyl laurate in supercritical carbon dioxide: comparison of two different enzymes

✍ Scribed by Shireesh Srivastava; Giridhar Madras

Book ID
102314475
Publisher
Wiley (John Wiley & Sons)
Year
2001
Tongue
English
Weight
74 KB
Volume
76
Category
Article
ISSN
0268-2575

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✦ Synopsis

Abstract

The enzymatic hydrolysis of p‐nitrophenyl laurate (PNPL) to p‐nitrophenol (PNP) was studied in supercritical carbon dioxide using crude Hog Pancreas Lipase (HPL) and P roqueforti Lipase (PRL). The two enzymes were compared for their optimal activity temperatures and reaction kinetics. HPL had a higher optimal temperature (65 °C) and better yield (22.5%) compared with PRL whose optimal temperature was 50 °C with a 15.3% yield. This is considerably higher than the optimum temperature of 37 °C and yields of less than 1% observed in the aqueous medium.

Β© 2001 Society of Chemical Industry

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