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Hydrolysis of monomeric substrates by porcine pancreatic (pro)phospholipase A2. The use of a spectrophotometric assay

โœ Scribed by J.J. Volwerk; A.G.R. Dedieu; H.M. Verheij; R. Dijkman; G.H. de Haas


Publisher
Elsevier Science
Year
2010
Tongue
English
Weight
803 KB
Volume
98
Category
Article
ISSN
0165-0513

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A continuous spectrophotometric assay fo
โœ William R. Snyder ๐Ÿ“‚ Article ๐Ÿ“… 1987 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 558 KB

A thiophosphate analog of dioctanoylphosphatidylcholine has been used as the substrate in a continuous spectrophotometric assay for the Bacillus cereus phospholipase C. The reaction has been monitored at 412 nm using 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) and at 324 nm using 4,4'-dithiopuridine