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Hydrolysis of cyclic nucleotides by a purified cGMP-stimulated phosphodiesterase: structural requirements for hydrolysis

โœ Scribed by Thomas Braumann; Christophe Erneux; Georg Petridis; Wolf-Dieter Stohrer; Bernd Jastorff


Book ID
113266242
Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
565 KB
Volume
871
Category
Article
ISSN
0167-4838

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The fluorescent 2'-methylanthraniloyl derivative of cyclic GMP undergoes a 45% decrease in fluorescence when it is cleaved by brain phosphodiesterase in the presence of calmodulin. This fluorescence decrease is dependent upon calcium, calmodulin, and phosphodiesterase, and correlates well (r = 0.996