A continuous fluorescence assay for cycl
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J.David Johnson; John D. Walters; John S. Mills
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Article
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1987
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Elsevier Science
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English
โ 376 KB
The fluorescent 2'-methylanthraniloyl derivative of cyclic GMP undergoes a 45% decrease in fluorescence when it is cleaved by brain phosphodiesterase in the presence of calmodulin. This fluorescence decrease is dependent upon calcium, calmodulin, and phosphodiesterase, and correlates well (r = 0.996