Hydrogen production using chloroplast membranes without oxygen scavengers: An assay with hydrogenases from aerobic hydrogen-oxidizing bacteria and flavodoxins fromDesulfovibriosp.
✍ Scribed by Thierry Lissolo; Marie-Françoise Cocquempot; Daniel Thomas; Jean LeGall; Klaus Schneider; Hans G. Schlegel
- Publisher
- Springer
- Year
- 1983
- Tongue
- English
- Weight
- 426 KB
- Volume
- 17
- Category
- Article
- ISSN
- 1432-0614
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✦ Synopsis
Electron carrier proteins from Desulfovibrio sp., especially flavodoxins, have been tested as electron mediator between spinach chloroplast membranes and hydrogenase from aerobic hydrogen-oxidizing bacteria. Due to the slow electron transfer between flavodoxin and hydrogenase the rate of hydrogen production is low.
However, the classical dramatic decrease of the hydrogen evolution rate in the absence of any oxygen scavengers is not observed when dealing with flavodoxin from Desulfovibrio desulfuricans strain 27774, 9 and the NAD-reducing hydrogenase from Nocardia opaca lb.