Hydrogen peroxide promotes transformation of rat liver non-neoplastic epithelial cells through activation of epidermal growth factor receptor

✍ Scribed by Ruo-Pan Huang; Ao Peng; Andre Golard; Moharmmad Z. Hossain; Ruochun Huang; Ya-Guang Liu; Alton L. Boynton

• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 220 KB
• Volume: 30
• Category: Article
• ISSN: 0899-1987
• DOI: 10.1002/mc.1030

✦ Synopsis

Abstract

Previous studies demonstrated that hydrogen peroxide (H~2~O~2~) is a tumor promoter in the rat liver epithelial cell line T51B. We investigated the pathway linking H~2~O~2~ to tumor promotion. H~2~O~2~ can directly induce tyrosine phosphorylation of epidermal growth factor receptor (EGFR). H~2~O~2~ and epidermal growth factor exerted similar effects on the induction of early growth response genes, disruption of gap junction communication, triggering of calcium inflow, and promotion of transformation. Furthermore, the effect of H~2~O~2~ on tumor promotion was blocked by abrogation of EGFR activation. Our results suggested that tumor promotion by H~2~O~2~ is mediated mainly through activation of EGFR in T51B cells. © 2001 Wiley‐Liss, Inc.