𝔖 Bobbio Scriptorium
✦   LIBER   ✦

HYDROGEN PEROXIDE INDUCES APOPTOSIS IN HUMAN HEPATOMA CELLS AND ALTERS CELL REDOX STATUS

✍ Scribed by Ji Li; Chung-Yang Huang; Rong-Liang Zheng; Kai-Rong Cui; Jian-Feng Li

Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
734 KB
Volume
24
Category
Article
ISSN
1065-6995

No coin nor oath required. For personal study only.

✦ Synopsis

Direct exposure of human hepatoma cell line SMMC-7721 to hydrogen peroxide (H2O2) can induce apoptosis. Apoptosis induced by H2O2 was inhibited by cycloheximide, actinomycin D, 3-aminobenzamide, EGTA or Zn2+. H2O2 can increase the level of intracellular Ca2+, downregulate GSH levels, slightly induce lipid peroxidation, and lead to change in the ratio of reduced ion components to oxidized ion components of cells. Analysis of flow cytometry indicates that H2O2 decreases the level of Bcl-2. The data indicate that H2O2-induced apoptosis requires new mRNA and protein syntheses; H2O2 can activate Ca2+/Mg2+-dependent endonuclease leading to internucleosomal DNA fragmentation and activation of poly (ADP-ribose) polymerase interfering with the energy metabolism of the cell. The H2O2 downregulation of GSH may be more important for apoptosis than H2O2 induction of lipid peroxidation, and the H2O2 induced changes in redox status of the cell may be among the original events which lead up to other biochemical changes.

📜 SIMILAR VOLUMES

Role of AKT kinase in sphingosine-induce
Role of AKT kinase in sphingosine-induced apoptosis in human hepatoma cells
✍ Hui-Chiu Chang; Li-Huang Tsai; Lea-Yea Chuang; Wen-Chun Hung 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 146 KB

## Abstract Our previous work has shown that a number of sphingolipid metabolites including sphingosine, sphinganine, and other long‐chain bases potently induced apoptosis in human hepatoma cells. In this study, we examined the possibility that sphingosine may trigger apoptosis in human hepatoma ce

Sodium selenite-induced oxidative stress
Sodium selenite-induced oxidative stress and apoptosis in human hepatoma HepG2 cells
✍ Han-Ming Shen; Cheng-Feng Yang; Choon-Nam Ong 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 French ⚖ 385 KB 👁 2 views

The mechanisms involved in the anti-carcinogenic activity of selenium remain to be elucidated. In the present study, we examined sodium selenite-induced oxidative stress and apoptosis in a human hepatoma cell line (HepG 2 ). Sodium selenite (10 M) exerted clear cytotoxic effect, as shown by the sign

Effector mechanisms of norcantharidin-in
Effector mechanisms of norcantharidin-induced mitotic arrest and apoptosis in human hepatoma cells
✍ Yan-Nian Chen; Jung-Chou Chen; Sui-Chu Yin; Guang-Sheng Wang; Wei Tsauer; Sheng- 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 French ⚖ 796 KB

## Abstract NCTD is a demethylated form of cantharidin with antitumor properties, which is now in use as a routine anticancer drug against hepatoma. However, there is limited information on the effect of NCTD on human cancer cells. In the present study, NCTD inhibited proliferation, caused mitotic

Histone deacetylase inhibitor trichostat
Histone deacetylase inhibitor trichostatin a induces cell-cycle arrest/apoptosis and hepatocyte differentiation in human hepatoma cells
✍ Yo-ichi Yamashita; Mitsuo Shimada; Norifumi Harimoto; Tatsuya Rikimaru; Ken Shir 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 French ⚖ 212 KB 👁 1 views

## Abstract Remodeling of the chromatin template by inhibition of HDAC activities represents a potential transcriptional therapy for neoplastic disease. A number of HDAC inhibitors that modulate __in vitro__ cell growth and differentiation have been developed. We analyzed the effects of TSA, a spec

Hydrogen peroxide–induced apoptosis medi
Hydrogen peroxide–induced apoptosis mediated by p53 protein in glial cells
✍ Yoshihisa Kitamura; Takashi Ota; Yasuji Matsuoka; Ikuo Tooyama; Hiroshi Kimura; 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 257 KB 👁 1 views

It is now generally accepted that massive neuronal death due to oxidative stress is a regular feature of brains in neurodegenerative diseases. However, much less attention has been given to the death of glial cells. In this study, we examined p53-sensitive apoptosis of cells by using human glioblast

2-methoxyestradiol induces apoptosis and
2-methoxyestradiol induces apoptosis and cell cycle arrest in human chondrosarcoma cells
✍ Yi-Chin Fong; Wei-Hong Yang; Sheng-Feng Hsu; Horng-Chaung Hsu; Kuo-Fung Tseng; C 📂 Article 📅 2007 🏛 Elsevier Science 🌐 English ⚖ 378 KB

## Abstract 2‐Methoxyestradiol (2ME) is an endogenous metabolite with estrogen receptor‐independent anti‐tumor activity. The current study seeks to determine the mechanism of anti‐tumor activity of 2ME on human chondrosarcoma. 2ME caused a time‐ and dose‐dependent cytotoxity in chondrosarcoma cells