Hydrogen exchange in nucleosides and nucleotides. Measurement of hydrogen exchange by stopped-flow and ultraviolet difference spectroscopy
โ Scribed by Cross, Dallas G.
- Book ID
- 126023599
- Publisher
- American Chemical Society
- Year
- 1975
- Tongue
- English
- Weight
- 715 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0006-2960
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๐ SIMILAR VOLUMES
A procedure to measure exchange rates of fast exchanging protein amide hydrogens by time-resolved NMR spectroscopy following in situ initiation of the reaction by diluting a native protein solution into an exchanging deuterated buffer is described. The method has been used to measure exchange rates
The hydrogen-isotope labeling of nucleic acid bases is sometimes required in nucleic acid research. Many papers have already appeared on deuteration of E-H in purines 2,3) and of 5-H in pyrimidines 4-6) through the ionic exchange processes. This paper describes catalytic hydrogen exchange of nucleos