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Hydrodynamic orientation of sperm heads for flow cytometry

✍ Scribed by Dean, P.N.; Pinkel, D.; Mendelsohn, M.L.

Book ID
118517548
Publisher
Biophysical Society
Year
1978
Tongue
English
Weight
350 KB
Volume
23
Category
Article
ISSN
0006-3495

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## Abstract When flat sperm heads that have been stained to fluoresce are examined in a flow cytometer, unexpectedly, skewed pulse height distributions are obtained despite the apparent homogeneity of the samples. This anomaly has been ascribed to an optical artifact that arises when the cells are