Hydration—dehydration-induced conformational changes of agarose, and kappa- and iota-carrageenans as studied by high-resolution solid-state 13C-nuclear magnetic resonance spectroscopy
✍ Scribed by Hazime Saitô; Motoko Yokoi; Junko Yamada
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 629 KB
- Volume
- 199
- Category
- Article
- ISSN
- 0008-6215
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✦ Synopsis
High-resolution, solid-state "C-n.m.r. spectra of agarose, and kappa-and iota-carrageenans were recorded for various states, including anhydrous and hydrated powder, film, gel, and lyophilized gel. The "C-n.m.r. spectra varied substantially with the extent of hydration. Fully hydrated samples, as in the agarose gels, gave rise to "C-n.m.r. peaks of the narrowest line widths, whereas dehydration caused considerable line-broadening or displacement of the Gl peak (or both). These observations were interpreted in terms of hydration-dehydration-induced conformational alteration or readjustment, depending on the extent of hydration or dehydration and the variety of samples used. "C-N.m.r. spectrometry of agarose gel by solid-state n.m.r. technique also indicated the existence of a rather rigid network structure with an anisotropic molecular tumbling.