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Hybridoma growth, metabolism, and product formation in HEPES-buffered medium: II. Effect of pH

✍ Scribed by Georg Schmid; Harvey W. Blanch; Charles R. Wilke

Book ID
105065801
Publisher
Springer Netherlands
Year
1990
Tongue
English
Weight
383 KB
Volume
12
Category
Article
ISSN
0141-5492

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The iron-rich (500 gM ferric citrate) protein-free supplement was added to six different basal media. Cell growth and monoclonal antibody production of a mouse-mouse hybridoma were investigated in 1.3 1 batch cultures performed in a laboratory bioreactor with automatic control of pH and dissolved ox