Hybridization Studies with Chiral Peptide Nucleic Acids

✍ Scribed by Gordon Lowe; Tirayut Vilaivan; Martin S. Westwell

• Book ID: 102564918
• Publisher: Elsevier Science
• Year: 1997
• Tongue: English
• Weight: 180 KB
• Volume: 25
• Category: Article
• ISSN: 0045-2068
• DOI: 10.1006/bioo.1997.1078

✦ Synopsis

A novel class of chiral peptide nucleic acids has been synthesized in which the sugarphosphate backbone of DNA has been replaced with the glycyl-proline backbone of both the L-and the D-configurations, nucleobases being attached through the 4-position of proline with cis-and trans-stereochemistry. The T 10 homopolymers with cis-stereochemistry in the Land D-series bind strongly to poly(dA) with T m values of 69 and 70ЊC, respectively. They bind more strongly to poly(rA) with T m values of 73 and 72ЊC, respectively, and with apparent 1 : 1 stoichiometry. Using a mixed sequence decamer it was found that the thermal stability of the chiral peptide nucleic acid/oligonucleotide complex was comparable to that formed by Nielsen's polyamide nucleic acid.