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Hybridization of the in vitro products of bacteriophage T7 RNA polymerase to restriction fragments of T7 DNA

โœ Scribed by William T. McAllister; Richard J. McCarron


Book ID
119009354
Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
877 KB
Volume
82
Category
Article
ISSN
0042-6822

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Random mutagenesis of the gene for bacteriophage T7 RNA polymerase was used to identify functionally essential amino acid residues of the enzyme. A two-plasmid system was developed that permits the straightforward isolation of T7 RNA polymerase mutants that had lost almost all catalytic activity. It