Hybridization kinetics on microarray surfaces
β Scribed by Val Golovlev; Ye Sun; Wen-hua Fan; Michael P. McCann
- Book ID
- 112011432
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 134 KB
- Volume
- 2
- Category
- Article
- ISSN
- 1860-6768
No coin nor oath required. For personal study only.
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Expression microarrays are often constructed by the immobilization of PCR products on two-dimensional modified glass slides or on three-dimensional microporous substrates. In this study we investigate whether the length of the immobilized species and the substrate choice influence hybridization dyna
The rate of hybridization of oligonucleotide target sequences to chemically immobilized oligonucleotide probes has been studied both with and without an electrical field. The probe size was 20-24 nucleotides (nt) while the target size ranged from 157 to 864 nt. In agreement with previous studies, co