Humoral immunity patterns based on antibody reactivity to rotavirus antigens in Brazilian children under 5 years of age
✍ Scribed by Andrade, Gildete P.; Lima, Lourdes R.A.V.; Hoshino-Shimizu, Sumie; Oliveira, Maria I.; Mendonça, Ronaldo Z.; Hársi, Charlotte M.; Stewien, Klaus E.
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 675 KB
- Volume
- 49
- Category
- Article
- ISSN
- 0146-6615
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✦ Synopsis
fants and young children, north eastern Brazil, gastroenteritis, immunoblotting of structural proteins, humoral immunity
The age distribution of antibody to simian rotavirus (SA-11) was studied in serum specimens obtained from 399 children aged to 5 years and living in the city of Recife (PE), located in the north eastern region of Brazil. Sera were examined for group-specific rotavirus antibody using a blocking enzyme immunoassay (bELISA) and a hemagglutination inhibition antibody (HIA) test, and for anti-VP2, anti-VP4, anti-VP6, and anti-VP7 antibodies using an immunoblotting assay (IBA). Antibody prevalence was similar in all bELlSA and HIA assays, showing a steep rise in the 6to 17-month-old age groups. The results indicate early acquisition of antibody to rotavirus. The majority of children aged 2 to 4 years had bELlSA (50% to 60%) and HIA (70% to 81%) antibodies. There was an association in prevalence data obtained by HIA and bELlSA with immunoblotting (IBA), revealing four serologic profiles. Children with profiles I and I 1 (60%) respectively had HA1 and ELISA antibody or HA1 antibody alone and all had immunoprotective antibodies to VP4 and/or VP7. These children were regarded as "immune," resembling convalescent patients with a rotavirus infection. Children with profile Ill (4%) had no HIA antibody and only non-protective anti-VP6 and/or VP7 antibody, and were considered to be "partially immune." Children with profile IV (36%) had no detectable antibody and were classified as "nonimmune." These children should be considered to be susceptible to rotavirus infection, with the riskof developing clinically severe diarrhea. o 1996 Wiley-Liss, Inc.