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Humoral and cell-mediated immune responses in humans to the NSP4 enterotoxin of rotavirus

✍ Scribed by Johansen, Kari; Hinkula, Jorma; Espinoza, Felix; Levi, Mikael; Zeng, Carl; Rud�n, Ulla; Vesikari, Timo; Estes, Mary; Svensson, Lennart

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
122 KB
Volume
59
Category
Article
ISSN
0146-6615

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✦ Synopsis

Rotavirus nonstructural protein NSP4 has recently been suggested to function as a viral enterotoxin and play a role in the pathophysiological mechanism whereby rotaviruses induce diarrhea. The ability of rotavirus NSP4 to stimulate a humoral immune response was examined in naturally infected children and adults, rotavirus vaccinated children, as well as a cellular immune response in adults. In this study, 10 of 10 naturally infected children and 9 of 10 rotavirusvaccinated children showed a weak humoral IgG immune response to recombinant NSP4 (rNSP4) and/or a synthetic peptide corresponding to residues 114-134 of NSP4. Modest serum IgG antibody responses were detected in 20 of 20 adults. A cellular immune response to rNSP4 and/or NSP4 114-134 were detected in 8 of 10 adults measured either as a T-cell proliferative response (7 of 10), an increased production of IL-2 (6 of 10), or increased production of interferon-␥ (8 of 10). These results indicate that NSP4 induces a humoral immune response in humans and show for the first time that NSP4 stimulates a cellular immune response, possibly including cytolytic T-cells.

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