Humanization of Lepidopteran Insect-Cell-Produced Glycoproteins
β Scribed by Tomiya, Noboru; Betenbaugh, Michael J.; Lee, Yuan C.
- Book ID
- 125941869
- Publisher
- American Chemical Society
- Year
- 2003
- Tongue
- English
- Weight
- 215 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0001-4842
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Stable transformation was used to generate a cloned insect cell line (Bm5 silkmoth cells) overexpressing human tissue plasminogen activator (tPA). This cell line expressed 135 Β΅g/mL single chain tPA in serum-free medium in static culture with a maximum specific activity of 120 IU/Β΅g. In serum-contai
An expression cassette for continuous highlevel expression of secreted glycoproteins by transformed lepidopteran insect cells has been developed as an alternative to baculovirus and mammalian cell expression systems. The expression cassette utilizes the promoter of the silkmoth cytoplasmic actin gen