HUMAN SPERM β-GLUCURONIDASE IS POORLY EXTRACTABLE BY TRITON X-100

✍ Scribed by ADRIANO BRANDELLI; PATRICIA V. MIRANDA; JORGE G. TEZÓN

• Publisher: Elsevier Science
• Year: 1996
• Tongue: English
• Weight: 121 KB
• Volume: 20
• Category: Article
• ISSN: 1065-6995
• DOI: 10.1006/cbir.1996.0040

✦ Synopsis

A part of sperm glycosidase activities was detected as detergent-insoluble after sequential extractions with Triton X-100. Sixty per cent of total -glucuronidase activity was found in the detergent-insoluble fraction. This portion of -glucuronidase was resistant to extractions in the presence of 1  KCl, chaotropic agents, colchicine or cytochalasine B, being only partially solubilized by 3  KCl or DNAse I treatment. Results demonstrate that -glucuronidase is tightly associated to the Triton X-100 resistant fraction.