Human skin penetration of a copper tripeptide in vitro as a function of skin layer

✍ Scribed by Jurij J. Hostynek; Frank Dreher; Howard I. Maibach

• Publisher: SP Birkhäuser Verlag Basel
• Year: 2010
• Tongue: English
• Weight: 206 KB
• Volume: 60
• Category: Article
• ISSN: 1420-908X
• DOI: 10.1007/s00011-010-0238-9

✦ Synopsis

Objective and design

Skin retention and penetration by copper applied as glycyl-l-histidyl-l-lysine cuprate diacetate was evaluated in vitro in order to assess its potential for its transdermal delivery as an anti-inflammatory agent.

Materials and methods

Flow-through diffusion cells with 1 cm^2^ exposure area were used under infinite dose conditions. 0.68% aq. copper tripeptide as permeant was applied on isolated stratum corneum, heat-separated epidermis and dermatomed skin and receptor fluid collected over 48 h in 4 h intervals using inductively coupled plasma mass spectrometry to analyze for copper in tissues and receptor fluid.

Results

The permeability coefficient of the compound through dermatomed skin was 2.43 ± 0.51 × 10^−4^ cm/h; 136.2 ± 17.5 μg/cm^2^ copper permeated 1 cm^2^ of that tissue over 48 h, while 97 ± 6.6 μg/cm^2^ were retained as depot.

Conclusions

Copper as tripeptide was delivered in potentially therapeutically effective amounts for inflammatory disease.