Human recombinant interferons-beta and -gamma decrease gelatinase production and invasion by human KG-2 renal-carcinoma cells

✍ Scribed by Kazuo Gohji; Isaiah J. Fidler; Rachel Tsan; Robert Radinsky; Andrew C. Von Eschenbach; Takashi Tsuruo; Motowo Nakajima

• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: French
• Weight: 734 KB
• Volume: 58
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910580313

✦ Synopsis

We investigated the effects of human recombinant interferons (r-IFNs) on gelatinase production and invasion by human renal-cell carcinoma (HRCC). Incubation of KG-2 HRCC with human r-IFN-P or -y (but not -a) suppressed transcription of the 72-kDa gelatinase gene and, hence, production of gelatinase activity. These inhibitory effects of interferons (IFNs) were independent of their antiproliferative effects. Treatment of KG-2 cells with r-IFN-P or -y significantly inhibited cell invasion through reconstituted basement membrane toward chemoattractants produced by kidney fibroblasts. The inhibitory activity of r-IFNs was specific to the KG-2 cells since gelatinase activity by various fibroblasts was unaffected. These findings suggest that r-IFN-P or IFN-y may be used to inhibit the invasive potential of HRCC.