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Human papillomavirus type 18 E6* MRNA in primary tumors and pelvic lymph nodes of hungarian patients with squamous cervical cancer

✍ Scribed by J. Czégledy; M. Evander; Z. Hernádi; L. Gergely; G. Wadell

Publisher
John Wiley and Sons
Year
1994
Tongue
French
Weight
868 KB
Volume
56
Category
Article
ISSN
0020-7136

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✦ Synopsis

Seven biopsy specimens from squamous-cell carcinomas of the uterine cervix were examined by RT-PCR for humanpapilloma-virus(HPV)-specific transcripts. With our HPVl8transcription-specific primer pair (5' nts 127-149; 3' nts 587-607), all 7 were shown to contain one strong viral mRNA signal from the early 6/early 7 open reading frames (E6/E7 ORFs). Sequence analysis of the cloned PCR product proved that the transcript was generated by splicing out an intron in E6 from nucleotides 233 to 4 16, thereby corresponding to the HPV I 8 E6* spliced mRNA. Nine out of 9 metastatic and 5 of 7

histologically negative lymph nodes from the same patients were also found to be positive for the same mRNA transcript. However, 4 HPV18 unrelated primary tumors and the connected regional pelvic lymph nodes (3 metastatic, 7 histologically negative) were negative for the HPV18 E6* mRNA.

Cytokeratin signals indicating tumor cells of epithelial origin

were detected in 7 out of the 9 transcript-positive lymph nodes with histological signs of metastasis and in 2 out of the 5 transcript-positive histologically negative lymph nodes. This suggests that the dispersion of the epithelial monoclonal tumor cells was lymphogenic in origin.

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