Human papillomavirus may be common within nasopharyngeal carcinoma of Caucasian Americans: Investigation of Epstein-Barr virus and human papillomavirus in Eastern and Western nasopharyngeal carcinoma using ligation-dependent polymerase chain reaction
✍ Scribed by Raj Punwaney; Margaret S. Brandwein; David Y. Zhang; Mark L. Urken; Ruinxue Cheng; Chang-Soo Park; Hong Bo Li; Xiaoping Li
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 112 KB
- Volume
- 21
- Category
- Article
- ISSN
- 1043-3074
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✦ Synopsis
Background. Nasopharyngeal carcinoma (NPC), particularly those tumors endemic to the Far East, commonly harbor Epstein-Barr virus (EBV), thought to serve as an important oncogenic promoter. Human papillomavirus (HPV) is associated with a proportion of upper aerodigestive tract carcinomas. We hypothesized that HPV might also contribute to the pathogenesis of NPC, and we queried whether geographic and racial distinctions may be identified between NPC of the Far East versus those diagnosed in Caucasian American patients with regard to the interrelationship of histologic subtype and viral infection.
Materials and Methods. Formalin-fixed paraffin-embedded tissue (FFPET) from 30 patients (6 Caucasian Americans, 1 Chinese American, 14 and 9 patients from Korea and China, respectively) were studied using the ligation-dependent polymerase chain reaction (LD-PCR). These cases were histologically classified according to the World Health Organization (WHO) schema for NPC. Consensus target probes complementary to the L1 region of over 30 HPV types, as well as target probes complementary to EBER-1 (EBV-related nontranslated latency-associated RNA), were used to amplify target sequences.
Results. Seven of 30 NPC (23%) contained HPV sequences. There were 6 Caucasian American patients with NPC; 3 cases (50%) were HPV positive (HPV+). Two of these Caucasian Americans had WHO type I tumors: one was HPV+ and EBV negative (EBV-) and the other was HPV-/EBV+. The remaining Caucasian American NPCs were WHO-II/III tumors which tested as follows: two were coinfected with HPV and EBV, the other two contained EBER but not HPV sequences. The single Oriental