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Human papillomavirus DNA in uterine cervix squamous cell carcinoma and adenocarcinoma detected by polymerase chain reaction

✍ Scribed by Akihiko Iwasawa; Pekka Nieminen; Matti Lehtinen; Jorma Paavonen

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 424 KB
Volume: 77
Category: Article
ISSN: 0008-543X
DOI: 10.1002/(sici)1097-0142(19960601)77:11<2275::aid-cncr14>3.0.co;2-u

No coin nor oath required. For personal study only.

✦ Synopsis

Substantial clinical, epidemiologic, and experimental evidence has reinforced the role of high risk human papillomavirus (HPV) types in the development of cervical carcinoma. The authors investigated HPV in the uterine cervix squamous cell carcinomas and adenocarcinomas of Finnish patients.

METHODS.

Specimens from 352 patients with uterine cervix squamous cell carcinomas and 108 with adenocarcinoma were examined for HPV DNA by polymerase chain reaction. The authors used consensus primers located in the L1 region, as well as HPV16, 18, and 33 type-specific primers located in the E6 region.

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