Aiistralin
DNA filter in situ hybridisation (FISH) was used to determine the presence of human papillomavirus (HPV) genotypes 6/11, 16118, and 31133 in cell scrapes of the cervix and vulva of 128 women who had precancerous lesions andlor HPV infection of the cervix diagnosed by cytology, colposcopy, and histology. HPV-DNA was found in 87 (68%) vulval and 95 (74%) cervical cell scrapes, and in both the vulval and cervical scrapes of 73 (57%) women, but not in either the vulva or the cervix of 19 women (15%). Of the HPV-DNA-positive smears, the prevalence of the HPV types was 61% HPV 16118, 14% HPV 6111, 3% HPV 31133, and 22% HPV 6111 and 16118. By contrast, HPV-DNA was not detected in the cervical smears of a control group of 35 women who were assessed t o be free of cervical abnormalities by colposcopy and cytology.
The epithelial response of the vulva and the cervix t o application of 5% acetic acid was assessed by colposcopy and the results correlated with the presence of HPV genotypes. A possible or definite disorder of the cervix and vulva was detected by colposcopy in 95 (74%) and 96 (75%) of the 128 cases, respectively. The colposcopic assessment of the vulva was inconclusive in ten cases (8%), and only eight women (So/,) were found to be free of both a vulval and cervical disorder.
This study shows subclinical papillomavirus infections of the vulva frequently coexist with HPV infections and precancerous lesions of the cervix. Careful colposcopic examination and greater attention to HPV infections of the vulva may improve the treatment and management of precancerous lesions and HPV infections of the cervix, and help t o reduce their recurrence.
Should new methods of treatment of HPV disorders be developed, vulval infections will need t o be treated coincidentally with those of the cervix.