Human melanoma cell-derived factor(S) stimulate fibroblast glycosaminoglycan synthesis

✍ Scribed by Michael Edward; Allison W. Grant; Rona M. Mackie

• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: French
• Weight: 720 KB
• Volume: 52
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910520327

✦ Synopsis

Conditioned media from cultures of human metastatic Hs294T melanoma cells contain a factor/facton that promote(s) fibroblast-mediated contraction of collagen lattices, and stimulate(s) fibroblast glycosaminoglycan (GAG) synthesis. Complete medium from melanoma cell cultures stimulated fibroblast hyaluronate synthesis 9.3-fold, and sulphated GAG synthesis 2.6-fold, as measured by 3H-glucosamine incorporation. )'SO4 incorporation into sulphated GAGS was essentially unaltered, the net result being a decrease in the degree of sulphation. Fibroblasts synthesized hyaluronate with an increased molecular weight when grown in the presence of the melanoma-cell culture medium, while the molecular weights of heparan and chondroitin sulphates remained essentially unaltered.

Our results indicate that the tumour-cell-derived factor(s) stimulate(s) changes in fibroblast glycosaminoglycan synthesis, and that these changes may facilitate tumour cell invasion in VNO.