Changes in shape, and aggregation that accompanies platelet activation, are dependent on the assembly and reorganization of the cytoskeleton. To assess the changes in cytoskeleton induced by thrombin and PMA, suspensions of aspirinβtreated,^32^Pβprelabeled, washed pig platelets in Hepes buffer conta
Human lymphocyte activation and differentiation in vitro induced by phorbol myristate acetate (PMA)
β Scribed by I. Sugawara; S. Ishizaka
- Publisher
- Elsevier Science
- Year
- 1982
- Tongue
- English
- Weight
- 73 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0192-0561
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The effects of phorbol myristate acetate (PMA) and dexamethasone on protein kinase C (PK-C) activity and eicosanoid production were characterized in primary cultures of rat glial cells. PMA (1,000 ng/ml) treatment for 2 hr resulted in a maximal effect (a 4-fold increase in PGE, production). Longer e
hepatocytes in coculture with activated PMNs. Thus, Polymorphonuclear leukocytes (PMNs) have been im-PMNs and hepatocytes produced NO in vitro, but neiplicated as cellular mediators of hepatic injury in models ther suppression nor elevation of NO production afof inflammation in vivo. In vitro, hepat