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Human liver alkaline phosphatase, purification and partial sequencing: Homology with the placental isozyme

โœ Scribed by Enrico Garattini; Jia-Cheng Hua; Yu-Ching E. Pan; Sidney Udenfriend


Book ID
115706939
Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
934 KB
Volume
245
Category
Article
ISSN
0003-9861

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Purification and partial characterizatio
โœ Per ร…ke Holmgren; Torgny Stigbrand; Gunhild Beckman ๐Ÿ“‚ Article ๐Ÿ“… 1977 ๐Ÿ› Springer ๐ŸŒ English โš– 461 KB

The I variant of placental alkaline phosphatase was purified to homogeneity by means of DEAE-cellulose chromatography, isoelectric focusing, and gel filtration on AcA-34. The specific activity of the I variant was found to be 3.33 micronkat/mg. The enzyme is a dimer with an isoelectric point of 4.6