Human intestinal mast cells produce IL-5 in vitro upon IgE receptor cross-linking and in vivo in the course of intestinal inflammatory disease
✍ Scribed by Axel Lorentz; Silke Schwengberg; Claudia Mierke; Michael P. Manns; Stephan C. Bischoff
- Book ID
- 101310893
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 115 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0014-2980
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✦ Synopsis
IL-5, known to be produced by T lymphocytes and eosinophils, is a key regulator of intestinal diseases such as parasitosis or eosinophilic gastroenteritis. Here we examined if mast cells contribute to the IL-5 production in human intestinal mucosa. The number of IL-5-positive lamina propria cells was substantially higher in patients with intestinal inflammatory diseases (5.3 ± 4.6 %, n = 17) compared to healthy controls (0.5 ± 0.9 %, n = 8, p X 0.01). In patients, the IL-5-positive cells were eosinophils (70 ± 13 %) and mast cells (29 ± 14 %), whereas in controls all IL-5-positive cells were eosinophils. IL-5-positive T cells were not detected, likely because they do not store IL-5. In vitro studies with isolated human intestinal mast cells and eosinophils showed that mast cells do not produce IL-5 constitutively, but release high amounts of IL-5 (315 ± 115 pg/10 6 cells) following IgE receptor cross-linking, compared to activated eosinophils (24 ± 5 pg/10 6 cells). Inhibitor studies suggest a regulation of IL-5 production at the transcriptional level. In conclusion our data demonstrate that activated mast cells are a potent source of IL-5 in the human intestinal mucosa.