𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Human interleukin-2 activated cytotoxic cells kill autologous glioma cellsin vitro

✍ Scribed by Vidar Bosnes; Henry Hirschberg

Publisher
Springer US
Year
1988
Tongue
English
Weight
504 KB
Volume
6
Category
Article
ISSN
0167-594X

No coin nor oath required. For personal study only.

✦ Synopsis

We have cultured peripheral blood lymphocytes (PBL) from glioblastoma patients in recombinant interleukin-2 (IL-2) containing medium for a period of 5 days. The cytotoxicity of these cells was tested on SaCr-labelled autologous dissociated glioblastoma cells which had not been cultured. Significant cytotoxicity against glioma cells was observed in seven out of nine cases. IL-2 activated PBL from normal donors were equally cytotoxic against these glioma cells. Autologous lymphocytes activated by phytohaemagglutinin were also lysed in most cases, and the erythroleukemia cell line K562 was highly susceptible to the cytotoxic capability of the IL-2 activated PBL. In cold target inhibition experiments, K562 inhibited the cytotoxicity against both autologous and allogenic glioma cells, and glioma cells inhibited the cytotoxicity against K562. Following immunomagnetic separation, the IL-2 activated cells demonstrated cytotoxicity against glioma cells, K562 cells, and PHA blasts in both the CD8 + and the CD8-subsets.

πŸ“œ SIMILAR VOLUMES

Induction of cytotoxic activity in human
Induction of cytotoxic activity in human lymphocytes against autologous and allogeneic melanoma cells in vitro by culture with interleukin 2
✍ P. Hersey; C. Bindon; A. Edwards; E. Murray; G. Phillips; W. H. McCarthy πŸ“‚ Article πŸ“… 1981 πŸ› John Wiley and Sons 🌐 French βš– 854 KB

## Abstract The influence of interleukin 2 (IL2) on the cytotoxic activity of lymphocytes from patients with melanoma against autologous and a variety of allogeneic melanoma cells was studied. IL2 was produced from blood lymphocytes cultured for 24 h with phytohaemagglutinin (PHA) and purified by m