Human iliac artery endothelial cells express both genes encoding the chains of platelet-derived growth factor (PDGF) and synthesize PDGF-like mitogen

✍ Scribed by N. M. Sitaras; E. Sariban; P. Pantazis; B. Zetter; H. N. Antoniades

• Publisher: John Wiley and Sons
• Year: 1987
• Tongue: English
• Weight: 552 KB
• Volume: 132
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041320228

✦ Synopsis

In human umbilical vein and bovine aortic endothelial cells in culture c-sis gene expression and secretion of platelet-derived growth factor (PDGF) has been previously demonstrated. We now report the presence of PDGF-1 and PDGF-Z/sis mRNA transcripts in primary cultures of human iliac artery endothelial cells (HIA-EC). Concomitantly, these cells synthesize and secrete PDGFlike proteins identified by direct irnmunoprecipitation with specific PDGF antiserum. T h e PDCF proteins secreted by HIA-EC have molecular weights of 31 and 35 kd under nonreducing conditions. Upon reduction these proteins are converted to the monomeric 15-and 16-kd forms. Conditioned media derived from HIA-EC stimulated the incorporation of 3H-thymidine by 3T3 cells and competed with 1251-PDCF for its binding to 3T3 cell membrane receptors. The biologic activity was stable to heating at 100°C for 10 min and sensitive to reducing agents, properties similar to those of authentic PDGF. Production of PDGF-like mitogen by the human arterial endothelial cells may play an important role in the paracrine modulation of arterial wall regeneration following vascular injury.