Human globin chain separation by capillary electrophoresis in acidic isoelectric buffers

✍ Scribed by Professor Pier Giorgio Righetti; Arianna Saccomani; Alexander V. Stoyanov; Cecilia Gelfi

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 533 KB
• Volume: 19
• Category: Article
• ISSN: 0173-0835
• DOI: 10.1002/elps.1150191034

✦ Synopsis

Human globin chain separation by capillary electrophoresis in acidic isoelectric buffers

A simple and reliable method, utilizing capillary electrophoresis in uncoated capillaries in acidic isoelectric buffers, is reported for screening for thalassemia and other defects on the synthesis of human globin chains. A solution of 50 mM iminodiacetic acid (PI 2.23), containing 7 M urea and 0.5% hydroxyethylcellulose (apparent pH 3.2) is used as background electrolyte for fast separation of heme-free, denatured globin (a, P and y) chains. Due to the low conductivity of such a buffer, high voltage gradients (600 V/cm) can be applied, thus reducing the separation time to only a few minutes. It is additionally shown that inclusion of 2 O/ o surfactant (Tween 20) in the background electrolyte induces the splitting of the y chains into two zones, called Ay and Gy, which represent the 136 of the chain.