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Human gingival fibroblast response to electropolished NiTi surfaces

✍ Scribed by Martha Es-Souni; Helge Fischer-Brandies; Mohammed Es-Souni

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
666 KB
Volume
80A
Category
Article
ISSN
1549-3296

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✦ Synopsis

Abstract

In the present study the in vitro biocompatibility of electropolished NiTi sheets is investigated. The assessment of cytotoxic effects due to potential Ni leaching from metal sheets was performed in direct contact with primary human fibroblast cultures using the 5‐bromo‐2′‐deoxyuridine cell proliferation assay and morphologic studies via light microscopy and scanning electron microscopy. To assess toxic effects related to Ni‐ions release, cells cultured in the presence of increasing concentrations of Ni^2+^ (NiSO~4~Β·6H~2~O) served as positive controls. It is shown that while the addition of NiSO~4~ caused severe proliferation decrease (˜80%) and morphologic damage at a concentration of 50 mg/L Ni^2+^ no negative effects were observed in fibroblasts cultured in the presence of electropolished NiTi sheets. The results are discussed in terms of surface topography effects on the biocompatibility of NiTi shape memory alloys. Β© 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2007

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