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Human fetal radial glia cells generate oligodendrocytes in vitro

✍ Scribed by Zhicheng Mo; Nada Zecevic

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
460 KB
Volume
57
Category
Article
ISSN
0894-1491

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✦ Synopsis

Abstract

Limited knowledge about human oligodendrogenesis prompted us to explore the lineage relationship between cortical radial glia (RG) cells and oligodendrocytes (OLs) in the human fetal forebrain. RG cells were isolated from cortical ventricular/subventricular zone and their progeny was followed in vitro. One portion of RG cells differentiated into cells of OL lineage identified by cell‐type specific antibodies, including platelet‐derived growth factor receptor‐alpha (PDGFRα), NG2, O4, myelin basic protein, and myelin oligodendrocyte glycoprotein. Moreover, using Cre Lox fate mapping (brain lipid binding protein‐Cre/Floxed‐yellow fluorescent protein) we established a direct link between RG cells and OL progenitors. In vitro generation of RG‐derived O4^+^ OL progenitors was enhanced by addition of sonic hedgehog (SHH) and reduced by the SHH inhibitor, cyclopamine, suggesting the role of SHH signaling in this process. In summary, our in vitro experiments revealed that a portion of cortical RG cells isolated from human forebrain at the second trimester of gestation generates OL progenitors and this suggests a role of SHH in this process. © 2008 Wiley‐Liss, Inc.

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